• To confirm iPS identity and purity we checked embryonic stem

  2018-10-20

  

  To confirm iPS identity and purity, we checked embryonic stem cell makers, Tra-1-81 (Fig. 2A) and Tra-1-60 (not shown) by live staining and found all order NS-398 are Tra-1-81 positive. Acknowledgment The project described was supported by Award Number I21BX002215 (WX) from the Biomedical Labor

• This study was approved by the Institutional

  2018-10-20

  

  This study was approved by the Institutional Stem Cell Research Oversight (ISCRO) committee in the Johns Hopkins University. Blood CD34+ vitamin d receptor were obtained with an informed consent from the patient. Materials and methods Acknowledgments We thank the patient for providing blood s

• br Results Relying on the high conservation of

  2018-10-20

  

  Results Relying on the high conservation of vertebrate TBX3 proteins, we used human TBX3 in murine ESCs due to its specific traceability. We inserted human TBX3 cDNA into the pEF-DEST51 vector (Invitrogen) for EF1α-promoter-based stable and constitutive overexpression in ESCs. Out of 20 independe

• ginsenoside rh2 Another remarkable observation of this study

  2018-10-20

  

  Another remarkable observation of this study is that ASCL1-iN ginsenoside rh2 are exclusively excitatory. This is surprising, because ASCL1 is not typically associated with the excitatory neuronal lineage during neural development (Bertrand et al., 2002; Fode et al., 2000; Guillemot et al., 1993; J

• Genetic manipulation by overexpression of exogenous factors

  2018-10-20

  

  Genetic manipulation by overexpression of exogenous factors such as Nanog, Klf2, and Prdm14 enables conversion of mouse EpiSCs to ESC-like cells (rESCs) (Gillich et al., 2012; Silva et al., 2009). Furthermore, transition of mouse EpiSCs to rESCs rarely occurs even after stimulation with LIF-STAT3 si

• prostaglandin receptor As the presence of NKX is

  2018-10-20

  

  As the presence of NKX6-1 is one of the distinguishing features of these two lineages, expression of this transcription factor is used to monitor the emergence of the beta cell lineage in hPSC differentiation cultures. Populations enriched in NKX6-1+ prostaglandin receptor will give rise to function

• The Bag KO mESC line

  2018-10-20

  

  The Bag1KO-mESC line maintains a classical dome-shaped ES cell morphology and expressed high levels of ES-associated protein such as, alkaline phosphatase (Fig. 1D). Immunofluorescent staining for the three key pluripotent stem cell markers Oct4, Sox2 and Nanog revealed that the Bag1KO-mESC line was

• br Conclusion The number of colonies at the first

  2018-10-20

  

  Conclusion The number of colonies at the first isolation step could only represent a more or less rich ECFC content of the donor but we demonstrated that the initial clonogenicity was also related to distinctive intrinsic stemness, proliferative and functional features. Moreover, our results enab

• Importantly expression of SMA is not simply

  2018-10-20

  

  Importantly, expression of α-SMA is not simply a marker for MF activation, but also the driver of cell function and fate. Increased expression of α-SMA directly reduces the clonal potential of hMSCs and guides their differentiation toward osteoblasts. Hence, α-SMA not only identifies osteoprogenitor

• br Experimental Procedures br Author Contributions

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction Mouse embryonic stem mdm2 p53 (mESCs) are cells derived from inner cell mass (ICM) that self-renew indefinitely in culture and can give rise to all somatic cell types both in vitro and in vivo (Evans and Kaufman, 1

• br Experimental Procedures br Author Contributions

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments We are deeply thankful to V. Fossati and N. Lamas for helpful critical reading of the manuscript and to J. Goldman for generously providing the O4 antibody. We thank J.-Y. Li for providing access to wild-type mice for deriving mE

• Our gene chip analysis also revealed

  2018-10-20

  

  Our gene chip analysis also revealed a robust upregulation of metallothionein (MT) family members that are involved in metal metabolism and detoxification, as well as reactive oxygen species (ROS) scavenging. Overexpression of MT has been shown to protect mice from streptozotocin (STZ)-induced β cel

• hcv protease inhibitor Main Text br Acknowledgments I have

  2018-10-20

  

  Main Text Acknowledgments I have been fortunate to work with tremendous mentors, colleagues, and students throughout my career and I am grateful for their past and current support. Research in my laboratory is supported by the NIH, the New York Stem Cell Foundation as a New York Stem Cell Founda

• The Gata site regulates a large

  2018-10-20

  

  The Gata2 +9.5 site regulates a large gene cohort in hemogenic endothelium, and these genes do not parse into a single pathway (Gao et al., 2013). The genes include Runx1, Lyl1, and Mpl, positive mediators of HSC generation and/or function. The identification of vital constituents of the +9.5-depend

• The CD and CD alleles

  2018-10-20

  

  The CD45.1 and CD45.2 ceramide differ by only five amino acids within the extracellular domain (Zebedee et al., 1991), resulting in epitope changes that permit specific recognition by monoclonal antibodies (Shen, 1981). The majority of the commonly used mouse strains express the CD45.2 allele. Back

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